Highly effective compact preparative system containing a UV-VIS DAD detector, a preparative gradient pump, an automatic inject valve and a fraction collector. The detector allows absorbance measuring at four wavelengths at the same time or scanning through entire spectrum

The system is controlled by a built-in PC and touchscreen.

A modular solution allows configuring the unit according to customer needs. This system contains a detector with a wavelength range of 200 –  800 nm, a pump with flow rate up to 50 ml/min and fraction collector.

Main parts

It is a UV-VIS diode array detector, which allows measuring the absorbance of four wavelengths in a range of 200–800 nm simultaneously in one cell just as scanning of whole spectra. Standardly is assembled with preparative cell PLCC07L, but other cells are available.

Fractions are collected according to chosen method, which is simply created using a touch screen. Racks for 8, 21,40 ml tubes, 60 ml vials or 12 funnels, 30 mm.

The valve is driven by a step motor and it is automatically switched by software. The system also allows manual sample injection by opening the column.

Installed industrial PC with Intel Atom 4 core processor. OS Linux, Touch screen 12,1“ 1024 x 786, SW QUEEN controls: gradient, flow rate, wavelengths, fractions collection, etc. Remote administration is available. Possibility of measuring at four wavelengths at the same time or scanning through entire spectra. Furthermore, the possibility of displaying 3D data.

Modified for the compact system, quaternary gradient pumps with flow rate up to 50 ml/min (30 MPa, 4351 PSI). Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.

Interface3xUSB, 2xLAN, RS232
Power supply100 - 240 V AC
Power input490 W
Dimensions W x H x D450 x 500 x 650 mm (17,72 x 19,69 x 25,59")
Weight55 kg (21,30 lb)
Interface3xUSB, 2xLAN, RS232
Power supply100 - 240 V AC
Power input400 W
Dimensions W x H x D500 x 678 x 482 mm (19.69 x 26.69 x 18.98")
Weight55 kg (121.30 lb)
Wavelength range 200 - 800 nm   (256 elements on CCD)
Number of channels (Signals)4
Scan200 - 800 nm,up to  20 Hz, step 1 nm
Typical spectral half-width10 nm
Accuracy of adjustment / Reproducibility± 1 nm / ±0.5 nm
Noise level at test cell (254 nm, TC 0.75s)± 5 x 10-5 AU
Drift at test cell (254 nm after 1 h)1 x 10-3 AU/hr
Materials in contact with mobile phaseFEP; fused silica, stainless steel, PEEK
Time constant (T63)0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1 s
Fraction collector 
 Available racks (two racks per unit)EC08 48 tubes of 8 ml
 EC21 36 tubes of 21 ml

EC40 24 tubes of 40 ml

 EC60 20 vials of 60 ml
 F12A 12 funnels, 30 mm

Maximum flow rate for tubes 8 ml

max. 50 ml/min
Maximum flow rate for tubes 21, 40 mlmax. 300 ml/min
Maximum flow rate for vials 60 mlmax. 300 ml/min
Maximum flow rate for funnels

max. 500 ml/min

Wetted materials FEP, Tefzel®, PPS, KEL-F, PTFE, PEEK, SS 316, glass SIMAX
Input tubingOD 1/8“ x ID 1/16“ FEP
Flow rate0.1 – 50 ml/min
Maximum operating pressure30 MPa (4351 PSI)
Flow rate setting 0.1 ml/min steps
Repeatability of flow rate adjusting± 0.5 %
Accuracy of flow rate setting± 2 %
Wetted materialsstainless steel, PEEK, TefzelTM, PE, ceramic, seals *
Output capillary outer diameter1/8”
Input tubing outer diameter1/8”
Gradient valves control module - OPTIONALLYUp to six valves